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Jia L. Song, Ph.D.
BISC 615 Developmental Biology
The potential for forming a new organism begins at fertilization, when the sperm meets the egg. Across species from the worm to the human, development of the newly fertilized egg to a juvenile or an adult requires the careful regulation of cell growth, differentiation, and morphogenesis. Different cell types make different sets of proteins, even when their genomes are identical. What makes each cell type unique is a direct result of differential gene expressions mediated by transcription factors and signaling molecules in response to chemicals and proteins in the cell and the environment. Dysregulation of important genes involved in developmental decisions can lead to human diseases. Our research addresses one of the key questions in developmental biology: How are genes regulated during early development?
To examine gene regulations in early development, we use the invertebrate sea urchin as a model organism. Since sea urchins belong to a sister group of chordates, their developmental processes have much in common with vertebrates and can provide invaluable information towards understanding the basic regulatory mechanisms underlying vertebrate development. The advantages of using these marine invertebrates to study early development are that millions of eggs or sperms are obtained from a single sea urchin (Fig.1), the developing sea urchin embryos are synchronous, transparent and easy to culture in the laboratory, they have a relatively simple body plan, and they are amenable to experimental manipulations.
Small RNAs, such as microRNAs, have been found to modulate the expression of thousands of genes that are involved in a broad range of biological processes, including cell proliferation and differentiation, apoptosis and metabolism, and development. Our overarching goal is to understand the regulatory roles of microRNAs in various developmental pathways.
Previously we identified four highly abundant microRNAs (miR-31, miR-1, miR-71, and miR-2012) that are important for conferring embryo survival upon knockdown of microRNA processing enzymes such as Dicer and Drosha. Currently we are taking an interdisciplinary approach using computational, proteomic, and experimental methods to identify and characterize these microRNA targets that are important for early development.
- Nadezda Stepicheva - Ph.D. student
- Santiago Suarez - MS student
- Priscilla Ahiakonu - MS student
- Tylor McCann-Undergraduate Researcher
- Priya Nigam - MS student 2013
- Archana Siddam -MS student 2012
- Undergraduate researcher with Honors Thesis: Lydia Bonar (2011), Megan Dumas (2013), Kelsie Landis (2014), Carissa McKinney (2014)
- Stepicheva, N., Nigam, P., Siddam, A., C. Peng, and J.L. Song. microRNAs regulate Beta-catenin of the Wnt signaling pathway in early sea urchin development. 2015 Jan (in press) http://dx.doi.org/10.1016/j.ydbio.2015.01.008
- Song, J.L. (2014) Broad distribution of ARF6 in somatic and germ cells of the sea urchin ovary. Molecular Reproduction and Development. March 29. doi: 10.1002/mrd.22326. PMID:24687463
- Stepicheva, N. and J.L. Song. High throughput microinjections of sea urchin zygotes. Journal of Visualized Experiments. 2014 Jan 21;(83). doi: 10.3791/50841.
- Yajima, M., Gustafson, E.A., Song, J.L, and G.M. Wessel. Piwi regulates Vasa accumulation during embryogenesis in the sea urchin. Developmental Dynamics. 2013 Nov 12. doi: 10.1002/dvdy.24096.
- Oulhen N, Yoshida T, Yajima M, Song JL, Sakuma T, Sakamoto N, Yamamoto T, Wessel GM. The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin. Dev Biol. 2013 Jan 25. doi: 10.1016/j.ydbio.2013.01.019. [Epub ahead of print]
- Song JL and Wessel GM. The forkhead transcription factor FoxY regulates Nanos. Mol Reprod Dev. 2012. Oct; 79(10):680-8.
- Song JL, Stoeckius M, Maaskola J, Friedlaender M, Stepicheva N, Juliano C, Lebedeva S, Thompson W, Rajewsky N, Wessel GM. Select microRNAs are essential for early development in the sea urchin. Dev Biol. 2012;362(1):104-13. Epub 2011 Dec 3.
- Wessel GM, Juliano CE, Wong J, Gustafson E, Song JL. Molecular markers of oocyte and primordial germ cell development in the sea urchin. Echinoderms. 2009:517‐528.
- Voronina E, Lopez M, Juliano CE, et al. Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development. Dev Biol. 2008;314(2):276–286.
- Song JL, Wessel GM. Genes involved in the RNA interference pathway are differentially expressed during sea urchin development. Dev Dyn. 2007;236(11):3180–3190.
- Sodergren E, Weinstock GM, Davidson EH, et al. The genome of the sea urchin Strongylocentrotus purpuratus. Science. 2006;314(5801):941–952.
- Song JL, Wong JL, Wessel GM. Oogenesis: single cell development and differentiation. Dev Biol. 2006;300(1):385–405.http://onlinelibrary.wiley.com/doi/10.1002/dvdy.24096/pdf
Education outreach at the Delaware Children Museum.
(A) Graduate student Stepicheva shows child and parent our animals for developmental studies.
(B) A child checks out the animals.
Collaboration with Professor Gallo-Fox in Early Childhood Education.
Phone: (302) 831-2794
Fax: (302) 831-2281
Office: 323 Wolf Hall
Lab: 018 Wolf Hall
Department of Biological Sciences
University of Delaware
Newark, DE 19716
- B.S. - Cornell University
- Ph.D. - University of Washington
- Postdoctoral - Brown University