Anja Nohe, Ph.D.

Research Interests

  • Development of Delivery Techniques. Current research is limited in tools to transfect proteins into cells, especially the cell nucleus. We recently developed a new very efficient transfection technique for proteins and nanoparticles into the cell nucleus. Our method provides 70-80 percent transfection efficiency of primary cells and cell lines. The uptake is very rapidly, cells stay alive for at least 3 days. Studies show that the method is non invasive, making it a powerful transfection tool. We are currently working on the detailed mechanism of the delivery.
  • Development of New Imaging Techniques. In order to study signal transduction in real time in live cells new imaging tools must be developed. One major goal of my research focuses on the further development of the Family of Image Correlation Spectroscopy (FICS), a powerful tool to measure protein dynamics, aggregation and signalling.
  • Differentiation of Stem Cells: Role of Hormones and Growth factors. In general my laboratory is interested in determining molecular dynamics underlying stem cell differentiation. In detail I am interested in developing new imaging techniques and probes to study real time dynamics of signal transduction mechanisms. We are especially interested to develop new probes and delivery techniques and use these approaches to investigate the influence of nanoscale receptor dynamics underlying diseases such as cancer and osteoporosis.

Current Projects

Differentiation of Stem Cells

  • Role of BMP in stem cell differentiation. We plan on identifying the role of our new identified proteins in BMP signaling and osteoblast differentiation. Moreover we want to test the effect of our developed peptides on Bone Marrow Stromal Cells isolated from mice exhibiting an age related osteoporotic phenotype. We expect that these peptides enhance osteoblast differentiation rather than fat cell differentiation. If this is the case these peptides could be potent new therapeutics for the treatment of osteoporosis.
  • Role of Vitamin D in Cancer. One of my current interests is to explore the molecular basis of cancer by establishing the role of 1,25 dihydroxy vitamin D3 (Vitamin D3) and its membrane receptor in hormone-dependent cancer cells, such as those of the breast, ovary, and prostate. In hormone-dependent cancers, tumor growth is driven by the binding of the hormone to its receptor. Vitamin D3 can have both preventive and therapeutic effects by regulating cell growth, the cell cycle, apoptosis, and differentiation Epidemiological studies have found a significant association between low serum levels and low dietary intake of Vitamin D3 and the incidence, degree of malignancy, metastases, and mortality of cancers of the breast, prostate, colon, and ovaries [1], [2]. Based on this research, it has been proposed that taking 1,000 international units (IU) - or 25 micrograms - of Vitamin D3 daily could lower an individual's cancer risk by 50% in colon cancer, and by 30% in breast and ovarian cancer. The mechanism for Vitamin D3's chemoprevention not well-defined, but understanding how it works would provide vital information for targeting populations at high risk for developing hormone-dependent cancers. Additionally, it could lead to improved chemotherapies that are more individualized and less toxic.

Research Group

Linda Sequeira (Research Associate)

Nguyen John (PhD student)

Weidner Hilary (PhD student)

Vrathasha Vrathasha (PhD student)

Lora Schell (Master student)

Daniel Halloran (Master student)

Ryan Wood (Undergraduate research)

Semaj Kelly (Undergraduate research)

Sabra Mahmoud (Undergraduate research)

 

Selected Publications

Schaefer RJ, Bonor JC, Joglekar MS, van Golen KL, Nohe AG: 1,25 Dihydroxyvitamin D3 uptake is localized at caveolae and requires caveolar function. J Biomed Nanotechnol 2013, 9:1707-1715.

Moseychuk O, Akkiraju H, Dutta J, D'Angelo A, Bragdon B, Duncan RL, Nohe A: Inhibition of CK2 binding to BMPRIa induces C2C12 differentiation into osteoblasts and adipocytes. J Cell Commun Signal 2013, 7:265-278.

Buchanan JL, Gilbert R, Ou Y, Nohe A, Schaefer R: The Kinetics of Vitamin D(3) in the Osteoblastic Cell. Bull Math Biol 2013, 75:1612-1635.

Utturkar A, Paul B, Akkiraju H, Bonor J, Dhurjati P, Nohe A: Development of Physiologically Based Pharmacokinetic Model (PBPK) of BMP2 in Mice. Biol Syst Open Access 2013, 2.

Bonor J, Reddy V, Akkiraju H, Dhurjati P, Nohe A: Synthesis and Characterization of L-Lysine Conjugated Silver Nanoparticles Smaller Than 10 nM. Adv Sci Eng Med 2014, 6:942-947.

Akkiraju H, Nohe A: Current Challenges in Bone Biology. Advanced techniques in biology & medicine 2015, 3:132.

Akkiraju H, Nohe A: Role of Chondrocytes in Cartilage Formation, Progression of Osteoarthritis and Cartilage Regeneration. J Dev Biol 2015, 3:177-192.

Akkiraju H, Bonor J, Olli K, Bowen C, Bragdon B, Coombs H, Donahue LR, Duncan R, Nohe A: Systemic injection of CK2.3, a novel peptide acting downstream of bone morphogenetic protein receptor BMPRIa, leads to increased trabecular bone mass. J Orthop Res 2015, 33:208-215.

Gangadharan V, Nohe A, Caplan J, Czymmek K, Duncan RL: Caveolin-1 regulates P2X7 receptor signaling in osteoblasts. Am J Physiol Cell Physiol 2015, 308:C41-50.

Kim HS, Kim JE, Frailey D, Nohe A, Duncan R, Czymmek KJ, Kang S: Roles of three Fusarium oxysporum calcium ion (Ca(2+)) channels in generating Ca(2+) signatures and controlling growth. Fungal Genet Biol 2015, 82:145-157.

Nohe A: Chalenges in Deciphering New Mechanisms in Cellular Biology. vol. 4: Biological Systems: Open Access; 2015.

Akkiraju H, Bonor J, Nohe A: An Improved Immunostaining and Imaging Methodology to Determine Cell and Protein Distributions within the Bone Environment. J Histochem Cytochem 2016, 64:168-178.

Akkiraju H, Bonor J, Nohe A: CK2.1, a novel peptide, induces articular cartilage formation in vivo. J Orthop Res 2017, 35:876-885.

Lisberg A, Ellis R, Nicholson K, Moku P, Swarup A, Dhurjati P, Nohe A: Mathematical modeling of the effects of CK2.3 on mineralization in osteoporotic bone. CPT Pharmacometrics Syst Pharmacol 2017, 6:208-215.

Nguyen J, Nohe A: Factors that Affect the Osteoclastogenesis of RAW264.7 cells. J Biochem Analyt Stud 2017, 2.

Nguyen J, Weidner H, Schell LM, Sequeira L, Kabrick R, Dharmadhikari S, Coombs H, Duncan RL, Wang L, Nohe A: Synthetic Peptide CK2.3 Enhances Bone Mineral Density in Senile Mice. Journal of Bone Research 2018 (accepted).

Swarup A, Weidner H, Duncan RL, Nohe A: A perfusion bioreactor to maintain bone cell viability ex vivo. Materials 2018 (accepted).

Vrathasha V, Booksh K, Duncan R, Nohe A: Time dependent internalization reveal the mechanism of cellular uptake   of quantum dot conjugates of CK2.3, a novel mimetic peptide that induces bone   formation in-vivo. Nanomaterials 2018, accepted.

 

Associate Professor

Phone: (302) 831-2959

Fax: (302) 831-2281

Email: anjanohe@udel.edu

Office: 324 Wolf Hall

Lab: 259 Wolf Hall

Address:
Department of Biological Sciences
Wolf Hall
University of Delaware
Newark, DE 19716

Education

  • Diplom. - University of Würzburg (Germany)
  • Ph.D. - Theodor Boveri Institute, University of Würzburg (Germany)
  • Postdoctoral - University of Western Ontario (Canada)