Salil A. Lachke, Ph.D.


BISC401 (Honors): Molecular Biology of the Cell (Course Website available through Canvas)

Research Interests

Second only to mental illnesses, we largely fear losing our sense of sight (Horowitz et al. 1997, Cepko 2001).  Loss of vision is detrimental and can result from a functional compromise of various components of the eye.  However, opacification of the transparent lens – clinically termed cataract – remains the leading cause of blindness in the world.  Cataract affects over millions of individuals worldwide and its incidence continues to rise with increase in human life expectancy.  Surgery remains the only treatment, carrying with it the risk of secondary cataract formation – an undesirable complication, especially in the elderly.  Therefore, strategies to prevent or delay the onset of this disease need to be designed, a challenge for which comprehensive understanding of the development and maintenance of this unique tissue is essential.

My laboratory uses systems-based approaches for the identification and functional elucidation of genes involved in organ development and disease. We have developed a bioinformatics-based approach termed "iSyTE" (integrated Systems Tool for Eye gene discovery, and have successfully used it to identify novel cataract genes and predict several uncharacterized genes with potential function in lens development.  Our goal is to characterize these candidate genes using biochemical, molecular, cell biological and genomics-level approaches.  As an important step towards this goal, we have used iSyTE to highlight the involvement of several new regulators, including TDRD7 (a post-transcriptional regulatory protein and RNA granule component) and CELF1 (a post-transcriptional regulatory protein and RNA-binding protein) in lens differentiation and cataractogenesis (Lachke et al. 2011, Science 331:1571-76 View in: Pubmed, Siddam et al. 2018, PLoS Genetics 14(3):e1007278 View in: Pubmed).  Our long-term goal is to deduce the gene regulatory networks (GRNs) that underlie organogenesis, which will need to be resolved in order to devise new regenerative therapies for diseases that affect these tissues.  Besides its clinical significance, we anticipate that insights gained from this research can potentially unveil basic regulatory principles relevant to the differentiation of other cell and tissue types.

Current Projects

Project 1:  Elucidate the function of cytoplasmic RNA-binding proteins and RNA granules in the lens.  iSyTE has predicted several uncharacterized lens-enriched components of RNA granules (RGs).  In this project, we seek to comprehensively define the different classes of RGs present in the developing lens.  We then aim to identify RNA targets of specific RG components and RNA binding proteins (RBPs) in the lens AEL and FCs.  This research is expected to make a major contribution to the field by generating binding motifs for several RBPs and by identifying their functional targets in the context of a developing tissue.


Project 2. Define the CELF1 and TDRD7 interactome and target RNAs in the lens.  In these projects, we aim to characterize the total repertoire of differentially regulated RNAs (including mRNAs and small RNAs) in Tdrd7 and Cefl1 null mutant lenses by expression profiling.  We also aim to define the CELF1 and TDRD7 lens interactome by using well-established proteomics-based protocols to identify the protein binding partners of CELF1 and TDRD7.  Identification of the critical nodes interacting with CELF1 and TDRD7 should have significant impact in providing insight into the post-transcriptional control of gene expression in lens development.

Project 3: Building a Gene Regulatory Network (GRN) for the developing lens.  We aim to use chromatin immunoprecipitation (ChIP), RNA-immunoprecipitation (RIP) coupled with expression analysis, protein binding microarrays, computational approaches and reporter analysis to define the nodes and edges functional in the lens GRN.  In our efforts to build a comprehensive GRN for the developing lens we seek to determine the regulators (transcription factors (TFs) and RBPs) and their target cis-Regulatory Modules (CRMs) that function to control lens expressed genes.

Watch a video summary of Dr. Lachke's research here.

Watch Dr. Lachke's lecture on the 2012 Nobel prize winning work of Dr. John Gurdon and Dr. Shinya Yamanaka here.

If you are an ARVO member, watch Dr. Lachke's presentation in the 2015 ARVO minisymposium "Integrated genomic networks in eye development and pathogenesis" here.

Watch Dr. Lachke's seminar in the Innovative Discoveries Series, at the Delaware Clinical & Translational Research ACCEL Program and the Christiana Care Value Institute here.

Read the NIH Director Dr. Francis Collins' comments on Dr. Lachke's research in the NIH Director's Blog

Lens Crafting

Research Group

Laboratory Members:

Salil Lachke (PI, Faculty Mentor)

Deepti Anand (Postdoctoral Fellow)

Salma Al Saai (Graduate Student)

Sandeep Aryal (Graduate Student)

Shaili Patel (Graduate Student)

Priyanka Ghorpade (Graduate Student)

Sanjaya Shrestha (Graduate Student)

Sadia Islam (Rotation Graduate Student)

Francisco Hernandez (Undergraduate Student)

Sabrina Luther (Undergraduate Student)

Juan Ruiz (Undergraduate Student)

Campbell Toensing (Undergraduate Student)

Lachke Laboratory Alumni:

Bailey Weatherbee (Undergraduate Honors Thesis 2016-2019) (Placement: *Gates Cambridge Scholar to pursue Doctoral degree with Dr. Magdalena Zernicka-Goetz at Cambridge University, Cambridge, England - *a highly competitive and prestigious scholarship awarded to select 34 students in 2019 across the United States)

Emily Paglioni (Undergraduate Research 2018-2019) (Placement: Associate Project Manager, Suvoda)

Kimia Ahmadizadeh (Graduated with M.S. 2017-2019) (Placement: Interviewing at Biotech companies)

Soma Dash (Graduated with Ph.D. 2013-2018) (Placement: Postdoc with Dr. Paul Trainor at Stowers Institute for Medical Research, Kansas City, MO - The Trainor lab is a leading lab in the world for craniofacial and neural crest cell research)

Dominic Villalba (Undergraduate Senior Thesis 2016-2018) (Placement: Research Technician with Dr. Raymond Penn at Sidney Kimmel Medical College at Thomas Jefferson University)

Archana Siddam (Graduated with Ph.D. 2012-2017) (Placement: *FDA (U.S. Food and Drug Administration) Commissioner's Fellowship - *a highly competitive and prestigious fellowship awarded to select 15 in 2017 across the United States)

Nathaniel Borders (Undergraduate Honors Thesis 2015-2017) (Placement: Research Assistant, Harvard Medical School, George Church Laboratory - the Church lab is at the forefront of biomedical technologies research in the world)

Joshua Barton (Undergraduate Honors Thesis 2014-2016) (Placement: M.D., Ph.D. Program, Sidney Kimmel Medical College at Thomas Jefferson University)

Anne Terrell (Research Associate II, 2013-2015) (Placement: Preceptor, University of Delaware)

Carrie Barnum (Graduated with M.S. 2012-2015) (Placement: Research Associate, University of Pennsylvania)

Sheila Sichani (Undergraduate Research 2014-2015) (Placement: Intern, Agilent Biotech)

Smriti Agrawal (Graduated with M.S. 2012-2014) (Placement: Doctoral Student, Baylor College of Medicine; had also recieved admissions in the doctoral programs at the University of Washington-Seattle and the University of California-Berkeley)

Christine Dang (Undergraduate Honors Thesis 2012-2014) (Placement: M.D. Program, Sidney Kimmel Medical College at Thomas Jefferson University)

Shaili Patel (Undergraduate Senior Thesis 2013-2014) (Placement: Research Associate, University of Pennsylvania)

Sylvie Smith (Undergraduate Student, Research 2014) (Placement: Associate Laboratory Technician, WuXi AppTec)

Stephanie Waters (Research Associate II, 2011-2013) (Placement: Associate Scientist, DuPont)

Selected Publications

  • 2019 (Total 10 or more expected: 7 published/in press/accepted, 4 in review/revision, 3 in prep.)
  • In Preparation (3)
  • Anand D, Djordjevic D, Kakrana A, Ramachandruni SD, Huang H, Ho JWK, Lachke SA. Evidence-based gene regulatory networks in organ development. Genesis 2019: In preparation
  • Anand D, Ramachandruni D, Huang H, Saadi I, Lachke SA. SysFACE: Systems tool for craniofacial expression based gene discovery. Human Genetics 2019: In preparation
  • Aryal S, Anand D, Hernandez FG, Weatherbee BAT, Wilmarth P, Reddy A, David LL, Lachke SA. MS/MS in silico subtraction-based profiling of the developing lens to facilitate cataract gene discovery. Human Genetics 2019: Preparated for submission
  • Submitted/In Review/Revision (4)
  • Dash S, Brastrom LK, Patel, SD, Scott CA, Slusarski DC, Lachke SA. The master transcription factor SOX2, linked to anophthalmia/microphthalmia, is post-transcriptionally regulated by the RNA-binding protein RBM24 in vertebrate eye development. Human Molecular Genetics 2019: In review
  • Viet J, Reboutier D, Hardy S, Lachke SA, Paillard L, Gautier-Courteille C. Modeling lens development and disease in Xenopus. Developmental Dynamics 2019: In revision
  • Barnum CE, Al Saai S, Cheng C, Anand D, Patel SD, Dash S, Siddam AD, Glazewski L, Paglione E, Polson S, Chuma S, Mason RW, Batish M, Fowler VM, Lachke SA. The Tudor-family protein TDRD7, mutated in congenital cataract, regulates the heat shock protein HSPB1 and fiber cell morphology. Human Molecular Genetics 2019: Sent for in-depth review, invited for re-submission after addressing reviewer comments
  • Padula SL, Anand D, Hoang T, Liu L, Liang C, Lachke SA, Robinson ML. The developing lens transcriptome depends on a counterbalance of Fgfr2- and Pten-regulated downstream signaling and transcription factor targets. Human Genetics 2019: minor revision
  • Published/In press/Accepted (7)
  • Weatherbee BAT, Barton JR, Siddam AD, Anand D, Lachke SA. Molecular characterization of the human lens epithelial cell line SRA01/04. Experimental Eye Research 2019: Accepted on August 30, 2019 View in: Pubmed
  • Sweat YY, Sweat M, Mansaray M, Cao H, Eliason S, Chalkley C, Anand D, Saadi I, Lachke SA, Butali A, Amendt B. Six2 regulates palatogenesis by inhibiting premature bone formation and a SIX2 variant is associated with cleft palate. Developmental Biology 2019: Accepted on August 23, 2019
  • Gowans LJJ, Cameron-Christie S, Slayton RL, Busch TD, Romero-Bustillos M, Eliason S, Sweat M, Sobreira N, Yu W, Kantaputra N, Wohler E, Adeyemo WL, Campbell C, Drummond BK, Markie DM, van Vuren WJ, Casamassimo PS, Ettinger R, Owais A, Anand D, Lachke SA, Staden IV, Amendt BA, Adeyemo AA, Murray JC, Robertson SP, Butali A. Missense pathogenic variants in KIF4A affect dental morphogenesis resulting in X-linked taurodontism, microdontia and Dens-invaginatus. Frontiers in Genetics 2019: in press View here
  • Cox TC, Lidral A, Liu H, Cox LL, Zhu Y, Moreno Uribe LM, Anand D, Deng M, Richter CT, Nidey NL, Standley JM, Blue EE, Chong JX, Smith JD, Kirk EP, Venselaar H, Krahn KN, van Bokhoven H, Zhou JH, Cornell RA, Glass IA, Bamshad MJ,  Nickerson DA, Murray JC, Lachke SA, Buckley MF, Roscioli T. Mutations in GDF11 and its binding partner, FST, as a cause of orofacial clefting in humans. Human Mutation 2019: published online June 18 doi: 10.1002/humu.23793 View in: Pubmed
  • Carlson JC, Anand D, Buxo CJ, Christensen K, Deleyiannis F, Hecht JT, Moreno LM, Orioli LM, Padilla C, Shaffer JR, Vieira AR, Wehby GL, Weinberg SM, Murray JC, Beaty TH, Saadi I, Lachke SA, Marazita ML, Feingold E, Leslie EJ. Phenotypic heterogeneity in orofacial clefts: a novel method for systematic genetic analysis and visualization of heterogeneity. Genetic Epidemiology 2019: published online June 6 doi: 10.1002/gepi.22214 View in: Pubmed
  • Ewans LJ, Colley A, Gaston-Massuet C, Gualtieri A, Cowley MJ, McCabe MJ, Anand D, Lachke SA, Scietti L, Forneris F, Zhu Y, Ying K, Walsh C, Lee E, Kirk EP, Field M, Miller D, Giunta C, Sillence D, Dinger ME, Buckley M, Roscioli T. Pathogenic variants in PLOD3 result in a Stickler syndrome-like connective tissue dysplasia with vascular complications. Journal of Medical Genetics 2019: published online May 25 doi: 10.1136/jmedgenet-2019-106019 View in: Pubmed
  • Butali A, Mossey PA, Adeyemo WL, Eshete MA, Gowans LJJ, Busch TD, Jain D, Yu W, Huan L, Laurie CA, Laurie CC, Nelson S, Li M, Sanchez-Lara PA, Magee WP 3rd, Magee KS, Auslander A, Brindopke F, Kay DM, Caggana M, Romitti PA, Mills JL, Audu R, Onwuamah C, Oseni GO, Owais A, James O, Olaitan PB, Aregbesola BS, Braimah RO, Oginni F, Oladele AO, Bello SA, Rhodes J, Shiang R, Donkor P, Obiri-Yeboah S, Arthur FKN, Twumasi P, Agbenorku P, Plange-Rhule G, Oti AA, Ogunlewe OM, Oladega AA, Adekunle AA, Erinoso AO, Adamson OO, Elufowoju AA, Ayelomi OI, Hailu T, Hailu A, Demissie Y, Derebew M, Eliason S, Romero-Bustillous M, Lo C, Park J, Desai S, Mohammed M, Abate F, Abdur-Rahman L, Anand D, Saadi I, Oladugba AV, Lachke SA, Amendt BA, Rotimi CN, Marazita ML, Cornell RA, Murray JC, Adeyemo AA.   Genomic analyses in African populations identify novel risk loci for cleft palate. Human Molecular Genetics 2019: 28:1038-1051 View in: Pubmed
  • 2018 (7)
  • Anand D, Kakrana A, Siddam AD, Huang H, Saadi I, Lachke SA. RNA-sequencing-based transcriptomic profiles of embryonic lens development for cataract gene discovery. Human Genetics 2018: 137:941-954 View in: Pubmed
  • Cox LL, Cox TC, Moreno Uribe LM, Zhu Y, Richter CT, Nidey N, Standley JM, Deng M, Blue E, Chong JX, Yang Y, Carstens RP, Anand D, Lachke SA, Smith JD, Dorschner MO, Quinlan A, Kirk E, Hing AV, Venselaar H, Valencia Ramirez LC, Bamshad MJ, Glass IA, Cooper JA, Haan E, Nickerson DA, van Bokhoven H, Zhou H, Krahn K, Buckley MF, Murray JC, Lidral AC, Roscioli T. Mutations in the epithelial cadherin-p120-catenin complex cause mendelian non-syndromic cleft lip and palate. American Journal of Human Genetics 2018: 102(6):1143-1157 View in: Pubmed
  • Krall M, Htun S, Anand D, Hart D, Lachke SA*, Slavotinek A*. A zebrafish model of foxe3 deficiency demonstrates lens and eye defects with dysregulation of key genes involved in cataract formation in humans. Human Genetics 2018: 137:315-328 View in: Pubmed *Co-corresponding authors
  • Siddam AD, Gautier-Courteille C, Perez-Campos L, Anand D, Kakrana A, Dang CA, Legagneux V, Mereau A, Viet J, Gross JM, Paillard L, Lachke SA. The RNA-binding protein Celf1 post-transcriptionally regulates p27Kip1 and Dnase2b to control fiber cell nuclear degradation in lens development. PLoS Genetics 2018: 14(3):e1007278 View in: Pubmed *Read comments on this paper by 2006 Nobel Laureate Dr. Craig Mello here
  • Anand D, Agrawal SA, Slavotinek A, Lachke SA. Mutation update of transcription factor genes FOXE3, HSF4, MAF and PITX3 causing cataracts and other developmental ocular defects. Human Mutation 2018: 39:471-494 View in: Pubmed *Featured on the Cover, see Cover Art here **Selected in Editor's Choice Articles, "considered particularly noteworthy by the journal's editorial board"

          Human Mutation Cover

  • Budak G, Dash S, Srivastava R, Lachke SA, Janga SC. Express: a database of transcriptome profiles encompassing known and novel transcripts across multiple development stages in eye tissues. Experimental Eye Research 2018: 168:57-68 View in: Pubmed
  • Kakrana A, Yang A, Anand D, Djordjevic D, Ramachandruni D, Singh A, Huang H, Ho JWK, Lachke SA. iSyTE 2.0: a database for expression-based gene discovery in the eye. Nucleic Acids Research 2018: 46:D875-D885 View in: Pubmed
  • 2017 (6)
  • Srivastava R, Budak G, Dash S, Lachke SA, Janga SC. Discovery of novel transcripts and splicing alterations during mammalian lens development. Scientific Reports 2017: 7:11572 View in: Pubmed
  • Wang Y, Terrell AM, Riggio BA, Anand D, Lachke SA, Duncan MK. Beta1-integrin deletion from the lens activates cellular stress responses leading to fibrosis and apoptosis. Investigative Ophthalmology & Visual Science 2017: 58:3896-3922 View in: Pubmed *Featured on the Cover

          IOVS Cover

  • Cavalheiro GR, Matos-Rodrigues GE, Zhao Y, Gomes AL, Anand D, Predes D, de Lima S, Abreu JG, Zheng D, Lachke SA, Cvekl A, Martins RAP. N-myc regulates growth and fiber cell differentiation in lens development. Developmental Biology 2017: 429:105-117 View in: Pubmed
  • Liu H, Busch T, Eliason S, Anand D, Bullard S, Gowans L, Nidey N, Saadi I, Dunnwal M, Lachke SA, Zhu Y, Adeyemo A, Amendt B, Roscioli T, Cornell R, Murray J., Butali A. Exome sequencing confirms the role of ARHGAP29 in mendelian orofacial clefting. Birth Defects Research Part A: Clinical and Molecular Teratology 2017: 109:27-37 View in: Pubmed
  • Patel N, Anand D, Monies D, Maddirevula S, Khan A, Algoufi T, Al-Owain M, Faqeih E, Alshammari M, Qudair A, Alshareef H, Aljubran F, Alsaif H, Ibrahim N, Abdulwahab F, Hashem M, Alsedairy H, Aldahmesh M, Lachke SA, Alkuraya FS. Novel phenotypes and loci identified through clinical genomics approaches to pediatric cataract. Human Genetics 2017: 136:205-225 View in: Pubmed
  • Anand D, Lachke SA. Systems biology of lens development: a paradigm for disease gene discovery in the eye. Experimental Eye Research 2017: 156:22-33 View in: Pubmed *Among the top 25 most downloaded articles between March-July 2016
  • 2016 (3)
  • Dash S, Siddam AD, Barnum CE, Janga SC, Lachke SA. RNA binding proteins in eye development and disease: implication of RNA granule components. Wiley Interdisciplinary Reviews: RNA 2016: 7:527-557 View in: Pubmed *Featured on the Cover, see Cover Art here

          WIREs Cover

  • Zhang Y, Fan J, Ho JWK, Hu T, Kneeland SC, Sellarole MA, de Vries WN, Lachke SA, Lang RA, John SWM, Maas RL. Crim1 regulates integrin signaling in murine lens development. Development 2016: 143:356-366 View in: Pubmed
  • Audette DS, Anand D, So T, Rubenstein TB, Lachke SA, Lovicu FJ, Duncan MK. Prox1 and fibroblast growth factor receptors form a novel regulatory loop controlling lens fiber differentiation and gene expression. Development 2016: 143:318-328 View in: Pubmed
  • 2015 (4)
  • Dash S, Dang CA, Beebe DC, Lachke SA. Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly. Developmental Dynamics 2015: 244:1313-1327 View in: Pubmed
  • Anand D, Agrawal SA, Siddam AD, Motohashi H, Yamamoto M, Lachke SA. An integrative approach to analyze microarray datasets for prioritiztion of genes relevant to lens biology and disease. Genomics Data 2015: 5:223-227 View in: Pubmed
  • Agrawal SA, Anand D, Siddam AD, Kakrana A, Dash S, Scheiblin DA, Dang CA, Terrell AM, Waters SM, Singh A, Motohashi H, Yamamoto M, Lachke SA. Compound mouse mutants of bZIP transcription factors Mafg and Mafk reveal a regulatory network of non-crystallin genes associated with cataract. Human Genetics 2015: 134:717-735 View in: Pubmed
  • Terrell AM, Anand D, Smith SF, Dang CA, Pathania M, Waters SM, Beebe DC, Lachke SA. Molecular characterization of mouse lens epithelial cell lines and their suitability for studying RNA granules and cataract genes. Experimental Eye Research 2015: 131:42-55 View in: Pubmed *Among the top 25 most downloaded articles between February-May 2015
  • 2014 (3)
  • Anchan RM, Lachke SA, Gerami-Naini B, Lindsey J, Ng N, Naber C, Nickerson M, Cavallesco R, Rowan S, Eaton JL, Xi Q, Maas RL. Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells. PLoS ONE 2014: 9(12):e115106 View in: Pubmed
  • Manthey AL, Terrell AM, Lachke SA, Polson S, Duncan MK. Development of novel filtering criteria to analyze RNA-sequencing data obtained from the murine ocular lens during embryogenesis. Genomics Data 2014: 2:369-374 View in: Pubmed
  • Manthey AL, Lachke SA, Fitzgerald PG, Mason R, Scheiblin DA, Duncan MK. Loss of Sip1 leads to migration defects and retention of ectodermal markers during lens development. Mechanisms of Development 2014: 131:86-110 View in: Pubmed
  • 2013 (2)
  • Wolf L, Harrison W, Jie H, Xie Q, Xiao N, Sun J, Kong L, Lachke SA, Kuracha MR, Govindarajan V, Brindle PK, Ashery-Padan R, Beebe DC, Overbeek PA, Cvekl A. Histone modifications and cell fate determination: lens induction requires CBP and p300. Nucleic Acids Research 2013: 41:10199-10214 View in: Pubmed
  • Tran P, Lachke SA, Stottmann R. Toward a systems-level understanding of the hedgehog signaling pathway: defining the complex, robust and fragile. Wiley Interdisciplinary Reviews: Systems Biology & Medicine 2013: 5:83-100 View in: Pubmed
  • 2012 (3)
  • Jumlongras D*, Lachke SA*, O'Connell DJ, Aboukhalil A, Li X, Choe SE, Ho JWK, Turbe-Doan A, Robertson E, Olsen BR, Bulyk ML, Amendt BA, Maas RL. An evolutionary conserved enhancer regulates BMP4 expression in developing incisor and limb bud. PLoS ONE 2012: 7(6):e38568 *Equal contribution View in: Pubmed
  • Lachke SA*, Ho JWK*, Kryukov GV*, O’Connell DJ, Aboukhalil A, Bulyk ML, Park PJ, Maas RL. iSyTE: integrated Systems Tool for Eye gene discovery. Investigative Ophthalmology & Visual Science 2012: 53:1617-1627. *Equal contribution View in: PubMed Presented at ARVO 2012, Abstract #1726 was Highlighted in "Emerging Trends and Hot Topics" by members of the Annual Meeting Program Committee as representing the newest and most innovative research being conducted in the various specialties; Abstract selected among the top ~5% of ~7000 abstracts and presentations in the meeting, View here
  • Lachke SA*, Higgins A*, Inagaki M, Saadi I, Xi Q, Long M, Quade BJ, Talkowski ME, Gusella JF, Fujimoto A, Robinson ML, Yang Y, Duong QT, Shapira I, Motro B, Miyoshi J, Takai Y, Morton CC, Maas RL. The cell-adhesion gene PVRL3 is associated with congenital cataract. Human Genetics 2012: 131:235-250. *Equal contribution View in: PubMed
  • 2011 (3)
  • Kasaikina MV, Fomenko DE, Labunsky VM, Lachke SA, Qiu W, Moncaster JA, Zhang J, Wojnarowicz Jr MW, Kumar SN, Malinouskil M, Schweizer U, Tsuji PA, Carlson BA, Maas RL, Lou MF, Goldstein LE, Hatfield DL, Gladyshev VN. 15 kDa selenoprotein (Sep15) knockout mice reveal roles of Sep15 in redox homeostasis and cataract development.  Journal of Biological Chemistry 2011: 286:33203-33212. View in: PubMed
  • Lachke SA, and Maas RL RNA granules and cataract. Expert Reviews of Ophthalmology 2011: 6:497-500 View in: PubMed
  • Lachke SA*, Alkuraya FS*, Kneeland SC*, Ohn T, Aboukhalil A, Howell GR, Saadi I, Cavallesco R, Yue Y, Tsai AC, Nair KS, Cosma MI, Smith RS, Hodges E, Alfadhli SM, Al-Hajeri A, Shamseldin HE, Behbehani A, Hannon GJ, Bulyk ML, Drack AV, Anderson PJ, John SW, Maas RL. Mutations in the RNA granule component TDRD7 cause cataract and glaucoma.  Science 2011: 331:1571-1576 (Research Article*Equal contribution View in: PubMed
  • 2010 (3)
  • Lachke SA, Zhang X, Maas RL. Photoreceptor cell development regulation. Encyclopedia of Life Sciences [John Wiley and Sons, Ltd: Chichester] 2010: Published June 19, DOI: 10.1002/9780470015902.a0000833.pub2 View in:
  • Rowan S*, Siggers T*, Lachke SA, Yue Y, Bulyk ML, Maas RL. Precise temporal control of the eye regulatory gene Pax6 through enhancer binding site affinity. Genes & Development 2010: 24:380-385 View in: PubMed Featured on Cover *Equal contribution

        Genes and Development Cover

  • Lachke SA*, Maas RL. Building the developmental oculome: systems biology in vertebrate eye development and disease. Wiley Interdisciplinary Reviews: Systems Biology & Medicine  2010: 2:305-323 Published online October 2, 2009 DOI: 10.1002/wsbm.59 *(Corresponding Author) View in: PubMed
  • 1999-2009 (10)
  • Burdon KP, Hattersley K, Lachke SA, Laurie KJ, Maas RL, Mackey DA, Craig JE. Investigation of eight candidate genes on chromosome 1p36 for total congenital cataract. Molecular Vision 2008: 14:1799-1804 View in: PubMed
  • Donner AL, Lachke SA, Maas RL. Lens induction in vertebrates: variations on a conserved theme of signaling events. Seminars in Cell & Developmental Biology 2006: 17:676-685 View in: PubMed
  • Brockert PJ*, Lachke SA*, Srikantha T, Pujol C, Galask R, Soll DR. Phenotypic switching and mating-type switching of Candida glabrata at sites of colonization. Infection & Immunity 2003: 71:7109-7118 *Equal contribution View in: PubMed
  • Lachke SA, Lockhart SR, Daniels KJ, Soll DR. Skin facilitates Candida albicans mating. Infection & Immunity 2003: 71(9):4970-4976 View in: PubMed Recommended by Faculty of 1000 as an Exceptional new finding
  • Lachke SA, Srikantha T, Soll DR. The regulation of EFG1 in white-opaque switching in Candida albicans involves overlapping promoters. Molecular Microbiology 2003: 48:523-536 View in: PubMed
  • Srikantha T, Lachke SA, Soll DR. Three mating type-like loci in Candida glabrata. Eukaryotic Cell 2003: 2:328-340 View in: PubMed Recommended by Faculty of 1000 as a new finding
  • Lachke SA, Joly S, Daniels K, Soll DR. Phenotypic switching and filamentation in Candida glabrata. Microbiology 2002: 148:2661-2674 View in: PubMed Featured on Cover

          Microbiology Cover

  • Ghormade VS, Lachke SA, Deshpande MV. Dimorphism in Benjaminiella poitrasii: involvement of intracellular endochitinase and N-acetylglucosaminidase activities in the yeast-mycelium transition. Folia Microbiologica 2000: 45:231-238 View in: PubMed
  • Lachke SA, Srikantha T, Tsai LK, Daniels K, Soll DR. Phenotypic switching in Candida glabrata involves phase-specific regulation of the metallothionein gene MT-II and the newly discovered hemolysin gene HLP. Infection & Immunity 2000: 68:884-895 View in: PubMed
  • Kvaal C, Lachke SA, Srikantha T, Daniels K, McCoy J, Soll DR. Misexpression of the opaque-phase-specific gene PEP1 (SAP1) in the white phase of Candida albicans confers increased virulence in a mouse model of cutaneous infection. Infection & Immunity 1999: 67:6652-6662 View in: PubMed

Other Information


2017 Elected to Phi Kappa Phi as Faculty Member, University of Delaware Chapter, Newark, DE, USA

Each year the UD Chapter invites select faculty to membership in recognitiion of their scholarship, teaching, and service to the University Community

2016 Gerard J. Mangone Young Scholar Award (Alison Young Professor), The Francis Alison Society, University of Delaware, Newark, DE, USA

Awarded to 1 UD Faculty Each Year for Excellence in Research and Teaching

2015 Frederick Bettelheim Award, National Foundation for Eye Research, Omaha, NE, USA

Awarded to 1 U.S. Lens Scientist biennially to attend the International Conference on the Lens

2015 Nominated by University of Delaware Provost Domenico Grasso for the 2016 Blavatnik Awards for Young Scientists, The New York Academy of Sciences, NY, USA

The Provost nominates 1 Scientist from the entire UD Life Sciences faculty for this competitive national award every year

2015 University of Delaware Research Foundation, Inc. Strategic Initiatives Award

Awarded to 4 UD Faculty

2014 Senior Thesis Mentor Award, University of Delaware, Newark, DE, USA

Awarded to 1 Faculty at Univ. of Delaware Each Year for Excellence in Mentoring Undergraduate Senior Thesis Research

2014 Knights Templar Eye Foundation, Inc. Pediatric Ophthalmology Career Starter Grant Award Competitive Renewal

Awarded to 9 Young Investigators in Vision Research Nationwide

2013 Kavli Frontiers of Science Fellow, The Kavli Foundation, Oxnard, CA, USA

Fellows are selected by a committee of US National Academy of Sciences members from among young researchers who have already made recognized contributions to science, including recipients of major national fellowships and awards and who have been identified as future leaders in science.

2013 Alcon Research Institute Young Researcher Grant Award

Awarded to 8 Young Investigators in Vision Research Worldwide

2013 Selected as Junior Member of the Alcon Research Institute, Forth Worth, TX, USA

2013 Knights Templar Eye Foundation, Inc. Pediatric Ophthalmology Career Starter Grant Award

Awarded to 19 Young Investigators in Vision Research Nationwide

2012 Pew Scholar in Biomedical Sciences, The Pew Charitable Trusts, Philadelphia, PA, USA

Awarded to “22 of America’s Most Promising Scientists”

2012 Fight for Sight Grant-in-Aid Award, Fight for Sight, Inc. New York, NY, USA

Awarded to 5 Young Investigators in Vision Research Nationwide

2012 University of Delaware Research Foundation, Inc. Award

Awarded to 11 UD Faculty

2010 Bettelheim Travel Fellowship, National Foundation for Eye Research, Omaha, NE, USA

Awarded to 1 young cataract researcher to participate in the XIX International Society for Eye Research Biennial Meeting

2009 Elected to Sigma Xi, full member Harvard Chapter

2009 BRI Research Excellence Award, Biomedical Research Institute and Brigham & Women’s Hospital, Boston, MA, USA

In recognition of Innovative Research

2008 Charles Epstein Trainee Research Award, American Society of Human Genetics

Excellence in Post-doctoral Clinical Research” Awarded to top ~1% from 100+ of national and international applicants

2005 D.C. Spriestersbach Prize, The University of Iowa in recognition of “Excellence in Doctoral Research”

Best Doctoral Dissertation in Life Sciences at the University of Iowa between the years 2003-2005; Awarded to one doctoral student within the entire Life Science community at University of Iowa every two years

2003 Developmental Studies Hybridoma Bank Sponsored Graduate Assistantship, The University of Iowa, Iowa City, IA, USA

2002 Award for Top Presentation, Jakobsen Graduate Student Forum, The University of Iowa, Iowa City, IA, USA

1999 Graduate Trainee Travel Award, American Society of Microbiology

1997 Scholarship Award, Sakal India Foundation, Pune, Maharashtra, India

“For pursuing graduate studies overseas


Articles in the University of Delaware community regarding research in the Lachke laboratory:



























Images of Funding Organizations


WIREs Cover



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Associate Chair
Associate Professor of Biological Sciences
Alumni Distinguished Early Career Professor of Biology
Gerard J. Mangone Scholar
Pew Scholar in the Biomedical Sciences

Phone: (302) 831-3040

Fax: (302) 831-2281


Office: 236 Wolf Hall

Lab: 351 Wolf Hall

Department of Biological Sciences
Wolf Hall
University of Delaware
Newark, DE 19716


  • B.Sc. - The University of Pune, India
  • M.Sc. - The University of Pune, India
  • Ph.D. - The University of Iowa, Iowa City
  • Postdoctoral - Harvard Medical School
  • Instructor - Harvard Medical School